Typhoid serology
Neutral resistance glass with funnel top.High-quality glass test tubes, and plastic test tubes are designed and constructed for optimal performance and excellent resistance to industrial scientific chemicals. Sturdy construction from borosilicate glass delivers a great range of operating temperatures. These precision-molded test and sample tubes are suited to a wide variety of applications.
Dreyer
mixer. Each Dreyer's tube was kept upright at room temperature and sealed until the time of the analysis. Each sample was then centrifuged for 10 min and the upper aqueous phase removed. The lower layer was made up to a total of 2 ml with chloroform, agitated for 60 s and placed into a Hewlett Packard quadrapol mass spectro-
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Widal test is a tube agglutination test employed in the serological diagnosis of enteric fever. The test is named after Georges Fernand Isidore Widal, a French physician and bacteriologist, born March 9, 1862, Algeria; died January 14, 1929, Paris. Principle: Patients' suffering from enteric fever would possess antibodies in their sera which.
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Widal Test is a Serological diagnostic tool, Widal test is done to detect Typhoid fever, a bacterial infection caused by Salmonella. The test is named after its inventor, Georges-Fernand Widal, a French physician who first described the use of serum agglutination to diagnose typhoid fever in 1896. The widal agglutination test is used to measure.
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(1) Dreyer's tube (narrow tube with conical bottom) for H agglutination and (2) Felix tube (short round-bottomed tube) for O agglutination. Now a days 3 x 0.5 ml Kahn tubes are used for both types of agglutination. Take 4 sets of 8 Kahn tubes/test tubes and label them 1 to 8 for O, H, AH and BH antibody detection.
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Here's a mnemonic which may come handy-. 1. Felix tube - Short round bottomed which detects the 'O' antigen. Remember the round bottom and round shape of the letter 'O'. 2. Dreyer's tube - Narrow, conical bottomed one which detects the 'H' antigen. Deserts are ' dry ' and pyramids, which resemble cones are in the deserts of Egypt.
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Dreyer's tube which is narrow tube with conical bottom for H agglutination. Similarly, Felix tube was used which is short round bottomed tube for O agglutination as second type of tube. Nowadays, Kahn tubes are used which is of size 3 x 0.5 ml for both types of agglutination.
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Conical bottom Dreyer's tube is used to see agglutination. 18. Role as virulence factor: The most important virulence factor responsible for endotoxic activity; it protects the bacteria from phagocytosis and bactericidal effect of complement. Makes the bacteria motile, hence contributing to their virulence. 19. Existence in phases: No phases
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Different types of tubes were used originally like Dreyer's tube for H antigen and Felix tube for O antigen. In modern test methods, Kahn tube or simple test tubes are used widely. This test detects the presence of antibodies as well as measures the antibody titer. The test is performed in the following steps:
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It is done by taking 0.2 mL of serum diluted 1:10 with distilled water in a Dreyer's tube and overlaying with a few drops of 4% solution of urea stilbamine. The formation of white flocculant precipitate indicates a positive test. The reaction is said to be more sensitive than the aldehyde test. Both the tests give false-positive reactions in.
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Each Dreyer's tube was kept upright at room temperature and sealed until the time of the analysis. Each sample was then centrifuged for 10 min and the upper aqueous phase removed. The lower layer was made up to a total of 2 ml with chloroform, agitated for 60 s and placed into a Hewlett Packard quadrapol mass spectrometer linked to a 5890 gas.
